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Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (5,517)
Protease Inhibitors and Phosphatase Inhibitors (3,819)
Magnetic Affinity Separation Particles (442)
Spin Columns and Gravity-flow Columns (342)
Cell Lysis Detergents (273)
Gel Filtration Media and Columns (168)
Miscellaneous Immobilized Affinity Ligands (114)
Cell Lysis Enzymes (108)
Biotin-Binding Affinity Resins and Beads (89)
Miscellaneous Immobilization Supports (89)
In vitro Protein Expression Systems (83)
Affinity Gels (77)

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226 – 240 of 10,282 results
226

Thermo Scientific™ DNase I Solution (2500 U/mL)

Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.

227

Invitrogen™ Ni-NTA Agarose

Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence

ENCOMPASS_PREFERRED
Type Agarose
Stationary Phase Ni-NTA
Content And Storage The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.
Form Suspension
Column Type Affinity Column
228

Lysozyme, MP Biomedicals™

Used for lysing E. coli and Streptomycetes for extraction purposes. Lysozyme, MP Biomedicals preferentially hydrolyzes the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. Enzyme activity: ≥20,000u/mg solid.

229
Promotions Available

Gibco™ AAV-MAX Lysis Buffer

AAV-MAX Lysis Buffer is a ready-to-use cell lysis reagent for the extraction of adeno-associated virus (AAV) particles from producer HEK293 cells. Cell lysis is detergent-based, eliminating the need for mechanical cell disruption.

ENCOMPASS_PREFERRED
230

Thermo Scientific™ Sodium Dodecyl Sulfate (SDS), C12

Highly purified form of SDS especially suited for protein experiments requiring tightly controlled solubility parameters.

231

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads
GSA_VA

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.

Particle Size 1 μm
Ligand Type Streptavidin
Type Magnetic Affinity Bead
Target Biotin
Content And Storage Upon receipt store at 4°C. Product is shipped on an ice pack.
Protein Form Recombinant
Form Liquid
Concentration Slurry: 10 mg/mL, 1% solids
Product Line Pierce™
232

Thermo Scientific™ Brij™-35, 30% Solution

Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.

233

Thermo Scientific™ Pierce™ Protein G Magnetic Beads
GSA_VA

Perform antibody purification and immunoprecipitation methods using manual or robotic magnetic separators with these high-capacity and high-throughput affinity particles.

Particle Size 1 μm
Ligand Type Protein G
Type Magnetic Affinity Bead
Target Antibody
Content And Storage Upon receipt store at 4°C. Product shipped with an ice pack.
Protein Form Recombinant
Form Liquid
Concentration Slurry: 10mg/mL, 1% solids
Product Line Pierce™
234

MilliporeSigma™ Calbiochem™ TGF-β RI Kinase Inhibitor VI, SB431542

TGF-β RI Kinase Inhibitor VI, SB431542, CAS 301836-41-9, is a cell-permeable inhibitor of SMAD2 phosphorylation. Inhibits the activity of ALK4 and ALK5 (IC50 = 140 nM and 94 nM, respectively).

235

Thermo Scientific™ Pierce™ NHS-Activated Magnetic Beads
GSA_VA

Immobilize proteins or other ligands for magnetic separation affinity purification with these magnetic particles coated with amine-reactive NHS-ester groups.

Particle Size 1 μm
Type Magnetic Affinity Bead
Target Amine
Content And Storage Upon receipt store at 4°C. Product shipped with an ice pack.
Protein Form Recombinant
Form Liquid
Surface Functionality NHS Ester
Concentration Slurry: 10 mg/mL, 1% solids
Product Line Pierce™
236

Thermo Scientific™ Tween™ 80 Surfact-Amps™ Detergent Solution

Thermo Scientific™ Surfact-Amps 80 Detergent Solution is highly-purified Tween-80 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Content And Storage Store in a cool, dry, and well-ventilated area
Form Liquid
Reagent Type Detergent Solution
Product Line Surfact-Amps™,Tween™
237

MP Biomedicals™ Deoxycholic Acid, Sodium Salt

Deoxycholic Acid, Sodium Salt is an anionic detergent used to solubilize membrane-bound proteins.

CAS 302-95-4
Form Powder
Product Type Detergent
Color White
Molecular Formula C24H39NaO4
Formula Weight 414.55
238

Gibco™ CTS™ AAV-MAX Lysis Buffer

Gibco CTS AAV-MAX Lysis Buffer is a ready-to-use cell lysis reagent for the extraction of adeno-associated virus (AAV) particles from producer HEK293 cells.

ENCOMPASS_PREFERRED
Content And Storage Store at 2–8°C.
Form Liquid
Product Type Cell Lysis Buffer
Reagent Type Cell Lysis Buffer
pH 6–8
Shelf Life 12 Months
Concentration 10X
Product Line AAV-MAX
239

Thermo Scientific™ POROS™ GoPure™ AAVX Pre-packed Column, 0.8 x 10 cm, 5 mL

Thermo Scientific™ POROS™ GoPure™ 0.8 cm pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) and hydrophobic interaction chromatography (HIC) resins or for small-scale purification.

240
Promotions Available

Gibco™ ExpiSf™ Protein Production Kit

Gibco™ ExpiSf™ Protein Production Kit is designed to significantly boost protein expression from high-density suspension cultures of Sf9 insect cells using the ExpiSf Expression System.

ENCOMPASS_PREFERRED
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
Expression Mechanism Cell-Based Expression
Format Liquid
Compatible Cells Insect Cells
Classification Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Expression System Baculovirus
Product Line ExpiSf™